Identification of quantitative trait loci for rice quality in a population of chromosome segment substitution lines.
نویسندگان
چکیده
The demand for high quality rice represents a major issue in rice production. The primary components of rice grain quality include appearance, eating, cooking, physico-chemical, milling and nutritional qualities. Most of these traits are complex and controlled by quantitative trait loci (QTLs), so the genetic characterization of these traits is more difficult than that of traits controlled by a single gene. The detection and genetic identification of QTLs can provide insights into the genetic mechanisms underlying quality traits. Chromosome segment substitution lines (CSSLs) are effective tools used in mapping QTLs. In this study, we constructed 154 CSSLs from backcross progeny (BC(3)F(2)) derived from a cross between 'Koshihikari' (an Oryza sativa L. ssp. japonica variety) as the recurrent parent and 'Nona Bokra' (an O. sativa L. ssp. indica variety) as the donor parent. In this process, we carried out marker-assisted selection by using 102 cleaved amplified polymorphic sequence and simple sequence repeat markers covering most of the rice genome. Finally, this set of CSSLs was used to identify QTLs for rice quality traits. Ten QTLs for rice appearance quality traits were detected and eight QTLs concerned physico-chemical traits. These results supply the foundation for further genetic studies and breeding for the improvement of grain quality.
منابع مشابه
Identification and Mapping of Quantitative Trait Loci Associated with Salinity Tolerance in Rice (Oryza Sativa) Using SSR Markers
Salinity stress is one of the most widespread soil problems next to drought, in rice growing areas. ReducingSodium (Na+), while maintaining Potassium (K+) uptake in rice are traits that would aid in salinity tolerance.Therefore, the identification of quantitative trait loci (QTLs) associated with those for Na+ and K+uptake, will enable breeders to use marker-assisted selection...
متن کاملTitle: Identification of Quantitative Trait Loci for Rice Quality in a Population of Chromosome Segment Substitution Lines
متن کامل
Identification of Genomic Regions and the Isoamylase Gene for Reduced Grain Chalkiness in Rice
Grain chalkiness is an important grain quality related to starch granules in the endosperm. A high percentage of grain chalkiness is a major problem because it diminishes grain quality in rice. Here, we report quantitative trait loci identification for grain chalkiness using high-throughput single nucleotide polymorphism genotyping of a chromosomal segment substitution line population in which ...
متن کاملLinkage analysis of microsatellite markers on chromosome 5 in an F2 population of Japanese quail to identify quantitative trait loci affecting carcass traits
An F2 Japanese quail population was developed by crossing two strains (wild and white) to map quantitative trait loci (QTL) for performance and carcass traits. A total of 472 F2 birds were reared and slaughtered at 42 days of age. Performance and carcass traits were measured on all of the F2 individuals. Parental (P0), F1 and F2 individuals were genotyped with 3 microsatellites from quail chrom...
متن کاملIdentification of major and minor genes associated with heading date in an indica × indica cross of rice (Oryza Sativa L.)
In this study, quantitative trait loci (QTLs) controlling rice heading date were detected in a F2:3 population derived from a cross between an indica rice variety, Tarom Mahalli, with early heading date, and an indica variety, Khazar, with late heading date. SSR linkage map was constructed using 74 polymorphic markers and 192 F2 individuals and covered a total of 1231.50 cM of rice genome. QTL ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of integrative plant biology
دوره 51 5 شماره
صفحات -
تاریخ انتشار 2009